The Study of Interaction among the Rare Earth、Drug and Biomacromolecule with Fluorescence Spectrophptometry and Its Application

The Study of Interaction among the Rare Earth、Drug and Biomacromolecule with Fluorescence Spectrophptometry and Its Application
　　Because of unique superiority of the rare earth fluorescence probe technology, it has been applied widely in the medicine analysis and the biological macro-molecule analysis aspect. In this paper, we established the determination methods for quinolones and the serum albumin using the rare earth fluorescence probe, and studied the interaction Segment neodymium magnets among drug and biomacromoleccule using fluorescence spectroscopy.
　　We have done the following work.(1)Used terbium (III) or europium (III) as fluorescents probe, we proposed a simple, rapid and sensitive new method for the determination of trace quinolones. Intermolecular energy transfer occurred in quinolones - terbium (III) or europium (III) complex and the fluorescence of terbium (III) or europium ( III) was sensitized by quinolones. Based on this fluoremetric methods for the determination of quinolones in pharmaceutical preparations were proposed and the determination limits achieved the ng level.Most biological fluids are fluorescent and provide high background fluorescence, which overlap the spectra of quinolone in most circumstance. Segment neodymium magnets However these spectra overlaps were successfully resolved by applying rare earth ion fluoresent probe techniques. So the fluoremetric method for the determination of the quinolones in human urine without tedious pre-separation was proposed. Proposed methods are simple, accurate and sensitive, and were applied satisfactorily to the determination of quinolones in its samples and the results