biologically active transition metal complexes and study design

Title: biologically active transition metal complexes and study designAuthor: Kou YingyingDegree-granting units: Nankai UniversityKeywords: chemical nuclease;; phenol schiff base;; polypyridyl;; transition metal complexesSummary:In recent years, artificial nucleic acid enzymes natural chemical and biological research has become the most active frontier areas. This paper focus on the field, the choice of more phenolic hydroxyl Schiff base and pyridine ligands as the main building components, supplemented by other ligands, synthesized 32 series transition metal complexes, its crystal structure analysis, elemental analysis and infrared applications spectroscopy and other methods were characterized; and apply UV, fluorescence spectroscopy, circular dichroism, and electrochemical methods and Magnetic lifter other means of agarose gel electrophoresis study its chemical nuclease activity. Part of the complex is also used EPR, variable temperature magnetic susceptibility was carried out by means of characterization and research.This thesis research are as follows:1 to phenolic hydroxyl Schiff base ligand design and synthesis of the five have not been reported for binuclear copper complexes. Relationship between the magnetic structure that complexes 1 and 4 ferromagnetic interactions, 3 and 5, compared with the anti-ferromagnetic interactions. Chemical nuclease activity results showed that: the existence of both redox agent, complexes all have significant chemical nuclease activity, the reaction mechanism of hydroxyl radical and singlet oxygen as the reactive oxidizing species, the cutting process.2, the design and synthesis of the five phenolic hydroxyl groups have not been reported binuclear cobalt manganese Schiff base complexes. They studied the chemical nuclease activity, the results show that in the presence of redox H_2O_2, in addition to complex 10, all but a chemical nuclease activity, oxidative cleavage reaction is the cutting process.3, the phenolic hydroxyl Schiff base ligand design and synthesis of ten mononuclear transition metal complexes. Chemical nuclease activity studies have http://www.999magnet.com/products/131-magnetic-lifter shown that the presence of the redox H_2O_2, all have complex chemical nuclease activity, the reaction mechanism of hydroxyl radical and singlet oxygen as the reactive oxidizing species, the cutting process.4 to polypyridyl ligands designed and synthesized for the seven single nuclear transition metal complexes. Study its interaction with DNA and DNA cleavage activity, the results show that the complexes are not a chemical nuclease activity.5, designed and synthesized five to phenanthroline dione and its derivatives as ligands of the copper complexes, of which 30 for the dual-core, the remaining four mononuclear complexes. Chemical nuclease activity studies have shown that complexes of DNA cutting reaction is singlet oxygen and hydroxyl radicals as reactive species in the oxidation of the cutting process. It also explores the complex relationship between the 30 of the magnetic structure was found between dual-core copper antiferromagnetic interaction.This paper mainly studied the phenolic hydroxyl Schiff base and polypyridyl type ligands as the main building components of transition metal complexes of chemical nuclease activity, expanding the chemical nuclease model system, the model complexes of nuclease activity was more in-depth research, calculate and get some quantitative parameters for the design and synthesis of efficient new, simple chemical structure of nucleic acid enzymes provide some valuable information.Degree Year: 2009