Novel Magnetic Nano-composites for molecular imprinting recognition of biological macromolecules

Title: Novel Magnetic Nano-composites for molecular imprinting recognition of biological macromoleculesAuthor: LinDegree-granting units: Nankai UniversityKey words: magnetic nanoparticles;; protein molecular imprinting;; poly-amino acid thin layer;; selective adsorptionSummary:Western blot was prepared with a specific protein on the template function of selective molecular imprinting technology. Performance limitations due to the protein, the relatively slow progress, technology is not yet mature, and still need to improve routine preparation and analysis methods to adapt to the physical properties of the protein (such as size, compatibility, Magnetic lifter conformational flexibility, water-soluble), in order to improve its stability, selectivity, site accessibility, and cost efficiency.This work combined with magnetic separation speed and selectivity of molecular imprinting technology, the advantages of pre-and nano-structured materials, large surface area and mass transfer speed advantages of using surface imprinting synthesis of four novel first average particle size of 100 ~ 400nm Core - shell imprinted nano-magnetic composite system, and for the identification of the separation of biological macromolecules. Four novel molecular imprinted nano-composite magnetic system includes: ① identify bovine hemoglobin core - shell magnetic nanoparticles molecularly imprinted polymers, namely the use of sol - gel prepared silica shell coated magnetic iron oxide nano- particles to amino acid as functional monomer and crosslinker, ammonium persulfate oxidation through the silica-modified iron oxide formed on the surface poly-amino acid molecular imprinted polymer thin; ② the expense of new Price bond bovine hemoglobin superparamagnetic nano imprinted polymer, that is, co-precipitation synthesis of Fe_3O_4 magnetic nanoparticles as the carrier, the silane-modified surface amino groups, aldehyde groups using glutaraldehyde double features to be modified magnetic nanoparticles, while the other end aldehyde groups and amino groups on proteins to imine covalent bonding, re-use of amino acid aggregation, synthesis of protein molecules of the surface imprinted core - shell superparamagnetic nano- polymer nanospheres; ③ novel magnetic surface imprinted bovine hemoglobin polystyrene nanoparticles coated with silica shells that the magnetic iron oxide nanoparticles through http://www.999magnet.com/products/131-magnetic-lifter silane reagent on the surface graft polymerization of polystyrene by surface imprinting synthesized by the larger size of the special surface morphology of the surface imprinted polystyrene super-paramagnetic nanoparticles; ④ superparamagnetic polystyrene of high dispersion, that is oleic acid and sodium dodecyl benzene sulfonate as a double Fe_3O_4 surfactant-modified magnetic nanoparticles, made of a stable aqueous dispersion of nano-magnetic fluid polymerizable Fe_3O_4 in Fe_3O_4 magnetic fluid in the presence of styrene and methyl methacrylate prepared by emulsion polymerization method of magnetic polymer nanospheres , then use the amino acid to its surface imprinting.By transmission electron microscopy, X-ray diffraction, thermal gravimetric analysis, vibrating sample magnetometer study of the molecular imprinting patterns of magnetic nanomaterials, adsorption, recognition performance, explores the molecular imprinted polymers for the template protein recognition mechanism. The results show that adsorption of poly-amino acid molecular imprinted polymer coated magnetic nanoparticles on the template protein with a high adsorption capacity and relatively low non-specific adsorption. These imprinted magnetic nanoparticles to overcome the whole protein molecule imprinted materials hinder the spread of the shortcomings of shell-specific recognition sites on a molecularly imprinted nanoparticles have special proteins on the template selectivity and high stability, and enables the protein molecules in a relatively spread within a short time to reach equilibrium, and the applied magnetic field for fast magnetic separation. These results demonstrate that the surface is imprinted polymer-modified superparamagnetic imprinted nanocomposites in chemical and biochemical separation, cell screening, biosensors, drug delivery and other areas with good prospects, and to achieve high abundance in the proteomics degree of protein removal and enrichment of low abundance proteins.Based on the above, this work combined with magnetic nano-technology, molecular imprinting technology, electromagnetism, the field of analytical chemistry, and many other advantages for the study of new multi-molecular imprinted polymer provides a new research ideas, and magnetic nanoparticles in molecular imprinting protein separation and enrichment of the application of the preliminary study.Degree Year: 2009